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化学合成siRNA
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基因 合成:  
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Application Examples of Gene Synthesis:

  1. Codon optimization to boost protein expression;
  2. Replace PCR cloning;
  3. Large-scale production of cDNA fragments for microarray chip;
  4. Clone humanized mouse antibodies or recombinant antibodies;
  5. Synthesize cDNAs for which the corresponding mRNA sources are difficult to obtain;
  6. Synthesize predicted genes/cDNAs;
  7. Synthesize genes or cDNAs that are difficult to clone;
  8. Synthesize alternatively spliced gene variants, SNPs, or any other variant type;
  9. Design gene therapy vector or genes;
  10. Design DNA vaccines;
  11. Design your own genes/cDNAs (deletion, mutation, and rearrangement etc.);
  12. Modify your gene for structure-function studies in enzymology and receptor biology.
>> 基因 合成
Size of Gene Turn around time Vector Quantity Price
0.5 -2 kb 10 to 18 pBluescriptII SK(+), pUC18 or 19 10 µg/plasmid $1.1USD/bp
2-3 kb 18 to 26 10 µg/plasmid $1.1USD/bp
3-5 kb 45 to 60 10 µg/plasmid $1.1USD/bp
5-10 kb inquire 10 µg/plasmid $1.1USD/bp
Our custom gene construction/synthesis service includes:
  1. Computer analysis of the sequence
  2. Polynucleotide synthesis
  3. Assembly of polynucleotides
  4. Cloning of the duplex fragment (gene) into your choice of vector
  5. Sequencing to verify the gene sequence
  6. Site specific mutagenesis, if necessary

Your DNA (minimum 10 µg) is delivered as a cloned gene along with a complete report. All custom work performed is completely confidential, and the resulting gene remains your sole property.

 
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